The discovery and engineering of novel fluorescent proteins (FPs) from diverse organisms is yielding fluorophores with exceptional characteristics for live‐cell imaging. In particular, the development of FPs for fluorescence (or Förster) resonance energy transfer (FRET) microscopy is providing important tools for monitoring dynamic protein interactions inside living cells. The increased interest in FRET microscopy has driven the development of many different methods to measure FRET. However, the interpretation of FRET measurements is complicated by several factors including the high fluorescence background, (...) the potential for photoconversion artifacts and the relatively low dynamic range afforded by this technique. Here, we describe the advantages and disadvantages of four methods commonly used in FRET microscopy. We then discuss the selection of FPs for the different FRET methods, identifying the most useful FP candidates for FRET microscopy. The recent success in expanding the FP color palette offers the opportunity to explore new FRET pairs. (shrink)

Alfred D. Chandler, Jr. is a worthy successor to Joseph Schumpeter as analyst of the large corporation and its role in economic growth. His new book, Scale and Scope, a comparative history of corporate capitalism in the U. S., Britain, and Germany, is animated by a vision of the large corporation as the leading force in economic growth, outdistancing older owner?managed forms of organization with a superior ability to invest entrepreneurially in large?scale production, mass distribution, and professional management. Chandler's account (...) implicitly relies on a dynamic?capabilities? theory of competition that reveals the fundamental irrelevance of neoclassical theory and policy, including antitrust policy. In some ways, however, Chandler's vision is too narrow, underplaying the role of markets in economic growth. One needs to know how economic systems build capabilities, not merely how the corporate institution does so. Moreover, there is reason to think that the last few decades have seen an organizational revolution with a dynamic rather different from the one that animates Chandler's account. As a result, Chandler's work is important for understanding the present?day issues of industrial competitiveness, but is only one piece of the puzzle. (shrink)

n the pioneering days of radio, my grandfather's job was to lecture to young engineers who were joining Marconi's company. To illustrate that any complex wave form can be broken down into summed simple waves of different frequencies (important in both radio and acoustics), he took wheels of different diameters and attached them with pistons to a clothesline. When the wheels went round, the clothesline was jerked up and down, causing waves of movement to snake along it. The wriggling clothesline (...) was a model of a radio wave, giving the students a more vivid picture of wave summation than mathematical equations could ever have done. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:The Object of French Studies GebrauchkunstRichard Klein (bio)If I may say something about the title—it points to the possibility that we are discussing the nature and future of French studies at the precise moment that France is about to disappear. There are those who believe that on January 1, 1999, when the euro becomes the common currency of the European Union, France will become a province of Germany. Effectively (...) real sovereignty will pass from Paris to Frankfurt.Already there are those at Cornell, for example, who think that we, in the French section, should seek to merge with the German department. I support the move (which isn’t going to happen), if only as a permanent reminder to avoid the fetishism of French. In today’s world, French is a question more than an answer to any question. French studies should be open even to the possibility that, these days, to speak French, it is becoming more and more necessary to speak German. The opposite has been true for a long time, as the French smugly know all too well—having for centuries lent their language of refinement and savoir-vivre to the Huns. The imminence of the conjuncture should remind us, too, that French studies is itself situated within the history of French—is transformed in its possibilities and embodiments by that history and by what is happening now, as we say, historically. Finally, the point here is to recall that the question of French studies has a history, that it is not merely being invented at this moment, but also reactivated as an object of discussion and debate.In fact, the current debate has been going on for some time. In 1993–94 the issue was addressed at two national conferences: a two-part symposium was organized by the Committee for the Future of French Studies at the University of Pennsylvania and the University of Wisconsin; another was held at New York University organized by the Association for French Cultural Studies and the Institute of French Studies. Summer institutes at Dartmouth and Northwestern have dealt with the issue. There is a large and growing bibliography. I cite some of the most prominent recent articles—to judge by how frequently they are cited in others I have read:—Emily Apter, “Comparative Exile: Competing Margins in the History of Comparative Literature.”—Lawrence Kritzman, “Identity Crises: France, Culture, and the Idea of the Nation.—Marie-Pierre Le Hir, “French Cultural Studies in the United States.”—Sandy Petrey, “French Studies/Cultural Studies: Reciprocal Invigoration or Mutual Destruction?”—Kristen Ross, Fast Cars, Clean Bodies: Decolonization and the Reordering of French Culture (often cited as the best example we have of what French studies might be).—Kristen Ross, “World Literature and Cultural Studies.”—Thomas Pavel, “Les études culturelles: Une nouvelle discipline?” Many questions have been raised in these articles about the possibility and necessity of doing French studies. There are those here who take it for granted that one can identify something French, can locate whatever it is that may be said to participate in a specifically French identity. Some, like Pavel, see French studies (and a fortiori so-called cultural [End Page 5] studies) as an excuse for doing n’importe quoi; it defies definition and merely authorizes more confusion and incoherence. Others, like Kritzman, have a more optimistic notion of how one could define French studies without abandoning some notion of the specificity and uniqueness, the irreducible identity of what is called French.Some critics, like Ross and Le Hir, tend to see French studies as inevitably serving to dissolve any particularism associated with French. What is called French culture has been irretrievably pluralized by the effects of decolonization, by the multiplication of francophonies and by their insertion into an Americanized system of globalization. Kids in suburbs of Paris and Cairo wear American baseball hats turned around and grow up eating McDonald’s. Where is the French in that? The object of French studies may be nothing but France’s assimilationist tendency to colonize others, to claim a universal mission, even as it is transformed by its own failed colonial history and by the very diversity it aims to... (shrink)

Robert Grosseteste was a prolific theological and scientific writer, a translator, bishop of Lincoln , and a candidate for sainthood. There have been several studies of his life and his works, the most recent being that of Richard Southern. Nevertheless, James McEvoy's comment in 1983 that “the course of Grosseteste's life up until 1225 is almost completely unknown” remains largely true. The problem is common in medieval history—there is a dearth of reliable sources for the subject's early life. I (...) do not intend to discuss here Grosseteste's youth, or even his life until 1198 . I will accept, with a few reservations, the model Southern presented for Grosseteste's life until 1198 — that Grosseteste came from an undistinguished family, acquired an education, and may have taught in local schools; after serving the bishop of Lincoln in a minor capacity, he managed to obtain an administrative position under Bishop de Vere in the diocese of Hereford. Unfortunately, Bishop de Vere died on Christmas Day 1198, and his household was disbanded. It is the period following 1198 until 1225 that needs scrutiny. The recent discovery of a reference to Robert Grosseteste in a thirteenth-century cartulary demands that we reconsider the way in which historians have structured Grosseteste's career, both because of what this manuscript records and because of the light it sheds upon the methodology that has underpinned Grosseteste scholarship. (shrink)

Henry Sidgwick remarked in The Methods of Ethics regarding pleasure that the “distinctions of quality that Mill and others urge may … be admitted as grounds of preference, but only in so far as they can be resolved into distinctions of quantity.” Sidgwick had not believed that Mill intended that resolution and commented in his history that “it is hard to see in what sense a man who of two alternative pleasures chooses the less pleasant on the ground of its (...) superiority in quality can be affirmed to take ‘greatest’ happiness or pleasure as his standard of preference.” Though Mill's view has been discussed rather often, the state of the criticism has more or less stabilized with Sidgwick's conclusion. (shrink)

The purpose of this paper is to introduce the multifaceted character of computer outliners and to describe some of their more distinctive features. The author explicates the way outlining processors might be productively employed in teaching philosophy and concludes the paper with a brief discussion on extant outlining software.

The purpose of this paper is to introduce the multifaceted character of computer outliners and to describe some of their more distinctive features. The author explicates the way outlining processors might be productively employed in teaching philosophy and concludes the paper with a brief discussion on extant outlining software.

This is a collection of essays touching on four distinct areas of interest to philosophers, lawyers, and political scientists: the philosophical justification for the adversary system; the problems of truth-finding in an adversarial setting; the issue of justice in relation to social policy-making; the right to privacy.

It is widely assumed that selection history accounts of function can support a fully reductive naturalization of functional properties. I argue that this assumption is false. A problem with the alternative causal role account of function in this context is that it invokes the teleological notion of a goal in analysing real function. The selection history account, if it is to have reductive status, must not do the same. But attention to certain cases of selection history in biology, specifically those (...) involving meiotic drive, shows that selection historical explanations are available in the case of items without any plausible function. Making contributions to goals, here fitness, must be introduced as an additional constraint in the analysis, either explicitly, or implicitly by appeal to individual functions or the selection-of/selection-for distinction. (shrink)

Two ideas govern the organisation of this collection. It is suggested that medieval philosophy is best studied as an interactive debate between thinkers of different times, and also the importance of the Ancient Greek philosophers in this field.

This book presents selections from the political and social thought of the ancient West from the early sixth century BCE up to the early years of the Roman Empire and includes not only the classic philosophers, Plato, Aristotle, and Cicero, but a number of dramatists and historians as well. The range of topics these writings treat run from class conflict, through the perils of democracy and the horrors of tyranny, to the place of women in politics, while the styles range (...) from the deeply dramatic of Sophocles' Antigone and the bawdy satire of Aristophanes' Assemblywomen to Plato's Socratic dialogue Republic and Aristotle's scientific treatise Politics. The translations have been chosen, and sometimes modified, for clarity and readability, and are accompanied by introductions which set forth the historical context and trace the general lines of thought the readings develop. Frequent notes explain references to ancient lore unfamiliar to many readers. Questions for discussion accompany each reading. (shrink)

In this important collection, the editors argue that medieval philosophy is best studied as an interactive discussion between thinkers working on very much the same problems despite being often widely separated in time or place. Each section opens with at least one selection from a classical philosopher, and there are many points at which the readings chosen refer to other works that the reader will also find in this collection. There is a considerable amount of material from central figures such (...) as Augustine, Abelard, Duns Scotus, and William of Ockham, as well as extensive texts from thinkers in the medieval Islamic world. Each selection is prefaced by a brief introduction by the editors, providing a philosophical and religious background to help make the material more accessible to the reader. This edition, updated throughout, contains a substantial new chapter on medieval psychology and philosophy of mind, with texts from authors not previously represented such as John Buridan and Peter John Olivi. (shrink)

In this book, Della Rocca traces out the conceptual links between key concepts and principles of Spinoza's system bearing on representation and the mind-body problem. In the course of doing so, he presents and defends a number of new, interesting theses about Spinoza's thought on these matters. The arguments are presented with impressive clarity and in great detail. All in all, the book is a significant contribution to the literature on Spinoza's metaphysics and epistemology, and should be read by anyone (...) with a serious interest in its historical subject or in the two perennial philosophical problems on which it focuses. (shrink)

From the neurophysiological perspective, thinking in dreaming and the quality of dream thought have been considered hallucinatory, bizarre, illogical, improbable, or even impossible. This empirical phenomenological research concentrates on testing whether dream thought can be defined as rational in the sense of an intervening mental process between sensory perception and the creation of meaning, leading to a conclusion or to taking action. From 10 individual dream journals of male participants aged 22–59 years and female participants aged 25–49 years, we delimited (...) four dreams per journal and randomly selected five thought units from each dream for scoring. The units provided a base for testing a hypothesis that the thought processes of dream construction are rational. The results support the hypothesis and demonstrate that eight fundamental rational thought processes can be applied to the dreaming process. (shrink)

This paper argues that Donald Davidson’s infamous denial in “A Nice Derangement of Epitaphs” that there is any such thing as a language, though it may not be fully supported by the arguments given for it in that paper, is nonetheless entailed by his semantic views generally, according to which the literal, linguistic meaning of a speaker’s words on an occasion is determined by how the speaker intended to be understood. In favor of this view, and thus against conventional languages, (...) the paper then argues that this understanding of linguistic meaning promises, in a way the conventional view of meaning does not, to make sense of linguistic normativity. (shrink)

Donald Davidson infamously claims that belief is in its nature veridical, and that skepticism is for this reason fundamentally incoherent. To those who take the issue of external world skepticism seriously, Davidson's arguments may seem to involve a conjuring trick. In particular, his invocation of an ‘omniscient interpreter’, whose intelligibility supposedly ensures that our beliefs must be largely true, has the air of incense and lantern-rubbing about it. Davidson's claim has received considerable critical response in the literature, almost all of (...) it negative. In my view, some commentators have indeed lit on a critical and controversial lemma in Davidson's argument, but this basic result has been obscured by being presented amidst an array of other criticisms that simply make no sense from a Davidsonian point of view. The aim of this paper is to clear away some of the confusion that stands in the way of a more productive evaluation of Davidson's important claim. (shrink)